How Is TLC Used In Forensics?

What are two specific tasks of a forensic odontologist?

Identify human remains that cannot be identified using face recognition, fingerprints or other means.

Identify bodies in mass fatalities, such as plane crashes and natural disasters.

Determine the source of bite mark injuries, in cases of assault or suspected abuse.

Estimate the age of skeletal remains..

Why silica gel is used in TLC?

Silica gel is by far the most widely used adsorbent and remains the dominant stationary phase for TLC. … The surface of silica gel with the highest concentration of geminal and associated silanols is favored most for the chromatography of basic compounds because these silanols are less acidic.

How is chromatography used by forensic scientists?

Background: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which they are made. … Forensic scientists are able to use ink chromatography to solve crimes by matching documents or stains found at a crime scene to the marker or pen that belongs to a suspect.

What does TLC mean?

Tender, Loving, CareIf you’ve ever spent some time on sites like or you’ve probably come across a house listing or two that states, the property needs some “TLC”. We aren’t talking about the all women rap group or a TV station, but Tender, Loving, Care. In other words, the property needs some repairs.

What is TLC in human body?

Total leucocyte count (TLC) or white blood cell count measures the number of leucocytes in the body. The differential leucocyte count (DLC) measures the percentage of each type of WBC present in the blood. A differential count also can detect immature WBCs and abnormalities.

What is the principle of TLC?

Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two phases between which they are to be partitioned. Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase).

How do police use chromatography?

In forensics, police use chromatography to identify and analyze substances found at a crime scene. Every mixture is made up of molecules of different chemicals, in varying amounts. Chromatography works by separating the chemicals out of a mixture and studying how the molecules behave during the separation process.

What does TLC stand for in forensics?

Thin Layer ChromatographyThin Layer Chromatography (or TLC) is a “tried and true” procedure that has been used for many years in drug identification laboratories.

What is the stationary phase in TLC?

Thin layer chromatography is done exactly as it says – using a thin, uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. The silica gel (or the alumina) is the stationary phase. … The mobile phase is a suitable liquid solvent or mixture of solvents.

How do you read a TLC plate?

In simple terms, this value is an indication of how far up a TLC-plate a compound has wandered. A high Rf -value indicates that the compound has travelled far up the plate and is less polar, while a lower Rf -value indicates that the compound has not travelled far, and is more polar.

Why do we saturate TLC chamber?

Damp, Chemical-Filled Air You want your chamber air thoroughly impregnated with solvent vapor in TLC because this keeps the stationary phase from drying out before the process is finished. The solvent evaporating from the paper saturates the chamber’s air so it doesn’t wick solvent off the stationary phase as quickly.

What is TLC used for in real life?

TLC can be used in forensic studies where body fluids, such as urine and blood can be tested for the presence of drugs. Acidic and neutral drugs can be identified by using octadecyl silica in the stationary phase, while plain silica and octadecyl silica can be used to identify basic drugs.

What type of chromatography is used in forensics?

Gas Chromatography (GC) Gas chromatography, or gas-liquid chromatography, involves using a liquid stationary phase and a gas mobile phase. In forensics, gas chromatography is used to determine if a deceased person has taken any alcohol or drugs prior to death, as well as determining if they had been poisoned.

Can I use a little TLC?

If someone or something needs some TLC, they need to be treated in a kind and caring way. TLC is an abbreviation for ‘tender loving care.

What does TLC Rf value mean?

retention factorThe retention factor, or Rf, is defined as the distance traveled by the compound divided by the distance traveled by the solvent. … The larger an Rf of a compound, the larger the distance it travels on the TLC plate.

What are the 4 types of chromatography?

There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography. Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers.

Why is it important not to use a pen when writing on the TLC plate?

Do not use excessive forces when writing on a TLC plate as this will remove the stationary phase. It is important to use a pencil rather than a pen because inks commonly travel up the plate with the solvent.

What does TLC means in a car?

Tender Loving CareIf you are going to buy a car you must give it means Tender Loving Care…..

What are the advantages of TLC?

Advantages of TLC include rapid analysis time because many samples can be analyzed simultaneously, low solvent usage on a per‐sample basis, a high degree of accuracy and precision for instrumental TLC, and sensitivity in the nanogram or picogram range.

Why is chromatography important in forensic science?

Chromatography can be advantageously used in forensic science for separation of compounds. At a crime scene, for example, chromatography can be used to determine if the dead person has alcohol or drugs or a poison in his/her body and this can be used to determine the cause of death.

What mixtures can be separated by chromatography?

Paper chromatography is a method for separating dissolved substances from one another. It is often used when the dissolved substances are coloured, such as inks, food colourings and plant dyes.